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In solid culture of Endomycopsis fibuliger No.55, Eudomuopsis javanensis No.112 and Candida tropicalis No.340, the conditions of enzyme (protease, amylase and cellulase) production and the influence of addition of (NH₄)₂SO₄were examined, and the results obtained were as follows. 10 Wheat bran medium is found to be the best on the enzyme production in case of simple material. The optimum conditions ; are water content added 100 to 120%, temperature 25 to 30℃ and incubation times 2 to 3 days. 2) The cellulase production was scarcely produced in the case of Endomyopsis fibuliger No.55, as well as, the amylase production was scarcely producted in the case of Endomycopsis javanensis No.112 and Candida tropicalis No.340. 3) The enzyme production was remarkably increased when 5% of (NH₄)₂SO₄as inorganic nitrogen sources was admixed to wheat bran. 4) When 5% of (NH₄)₂SO₄was admixed to medium, the ratio of protein increase was 10.2 to 17.7% in wheat bran medium and 10.6 to 17.9% in sweet potato cake medium.
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